FIG. 1.
Immunization scheme and timeline. The vaccine components for each immunization group are shown in Table 1. Groups receiving a DNA prime and FI-HSV2 (or FI-Mock) boost (groups 1 to 4) were i.d. immunized between weeks 0 and 5 and then s.c. boosted on weeks 11 and 15. The gD2 subunit control group (gD2 su) received s.c. injections concurrently with groups receiving FI-HSV2 boosts. Each vaccine group consisted of 10 guinea pigs. The HSV-2 challenge (6 × 105 PFU) was given intravaginally (i.vag.) at week 18. The acute disease phase was defined as up to 14 days postchallenge (weeks 18 to 20), and the recurrent phase was defined as days 15 to 100 postchallenge (to week 33). Guinea pigs were sacrificed, and the dorsal root ganglia (DRG) were harvested over the last 2 weeks of the experiment. The arrows show the weeks of test bleeds for antibody determination.