FIG. 4.
Protection against acute-phase vaginal HSV-2 shedding. On the days shown postchallenge, each guinea pig was swabbed intravaginally, and the swabs were placed in 1 ml of DNG and frozen until standard plaque assay on Vero cells as described in Materials and Methods. (A) Levels of vaginal virus in the swabs are shown as group mean log10 PFU, and error bars represent the standard deviations (n = 10 for all groups except for the UL5, UL30, gD2t-FI-HSV2 group where n = 9). The dotted line shows the limit of assay sensitivity. (B) Kinetics of viral clearance are represented as the percentage of guinea pigs in each group shedding virus on the indicated days postchallenge.