FIG. 5.
Viral and host protein syntheses are affected by the amino-terminal mutations. BHK-21 cells infected at an MOI of 10 PFU/cell were labeled with [35S]cysteine and methionine at 3, 7, and 11 h (A) or 7, 17, and 23 h (B). After 1 h of labeling, cells were harvested and postnuclear lysates were separated by SDS-polyacrylamide gel electrophoresis and visualized by a phosphorimager. (C) Viral protein accumulation. Unlabeled postnuclear lysates were harvested at 8 hpi. Separated proteins were transferred to nitrocellulose and probed with the indicated antisera. Bands were visualized by chemiluminescence. α, anti.