TABLE 1.
Titers and plaque morphology of nsP4 amino-terminal mutants
| Virus | Plaque size (mm) | Titer (PFU/ml) |
|---|---|---|
| wt | 4 | 1 × 109 |
| D6A | 3.5 | 8 × 108 |
| D6K | 3.5 | 1 × 108 |
| P9A | 4 | 1 × 109 |
| GPG(8-10)VAVa | 0.5 | 3 × 107 |
| H11A | 3.5 | 1 × 109 |
| H11E | 3 | 5 × 108 |
| K15A | 4 | 1 × 109 |
| QKK(13-15)AAA | 4 | 5 × 108 |
| E24R | 4 | 1 × 109 |
| E33A | 4 | 1 × 109 |
| E33R | 4 | 1 × 109 |
| H36A | 4 | 1 × 109 |
| APV(37-39)ERE | 2 | 5 × 108 |
| D41Aa | 1 | 2 × 108 |
| D41Ka | 4 | 1 × 109 |
| K44A | 4 | 1 × 109 |
| KEE(44-46)AAA | 4 | 4 × 108 |
| R51A | 4 | 1 × 109 |
| MMP(54-56)KKN | 2 | 5 × 108 |
| R63A | 4 | 1 × 109 |
| KSR(61-63)AAA | 4 | 1 × 109 |
| RK(67-68)AA | 3.5 | 1 × 109 |
| K73A | 3.5 | 8 × 108 |
| K73D | 3 | 4 × 108 |
| R79A | 4 | 1 × 109 |
| G83La | 2.5 | 5 × 108 |
| Y87A | 3 | 8 × 108 |
| P94V | 4 | 1 × 109 |
| Y97A | 3 | 5 × 108 |
a
Mutants used in subsequent studies.