FIG. 1.

Expression of PrP glycosylation mutants in Rov cell cultures. Immunoblotting analysis with 4F2 antibody of Rov cells that expressed PrP mutants with asparagine substitutions at codon 184 or 200 (sheep sequence numbering) or at both codons (double mutants) is shown. The positions of the diglycosylated, monoglycosylated, and unglycosylated forms of PrP are indicated on the left. While diglycosylated species predominate with wild-type (WT) PrP, monoglycosylated species become the major component with single mutants. Double mutants show only one band corresponding to unglycosylated PrP. Molecular weights (in thousands) are indicated on the right.