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Prion infection of Rov cells expressing PrP glycosylation mutants. Cultures exposed to an infectious inoculum of the 127S ovine prion strain were tested for the presence of PK-resistant PrP by immunoblotting using Sha31 antibody. Results of a typical experiment are shown, and the number of passages postexposure is indicated. The gels were loaded with 250 μg or 25 μg of PK-digested proteins per lane for the mutant or wild-type PrP-containing samples, respectively.
