TABLE 1.
Characteristics of the PrP glycosylation mutants
| Glycotype | Genotype | Cell surface expressiona | Conversion |
|---|---|---|---|
| Wild type (VRQ) | Wild type | + | + |
| Monoglycosylated site 1 mutants | N184D | + | + |
| N184H | + | − | |
| N184Q | + | − | |
| N184S | + | − | |
| N184T | + | − | |
| N184G | + | − | |
| N184E | + | − | |
| T186A | − | − | |
| T186N | − | − | |
| Monoglycosylated site 2 mutants | N200D | + | + |
| N200H | + | + | |
| N200Q | + | + | |
| N200S | + | + | |
| N200T | + | + | |
| Unglycosylated double mutants | N184D-N200D | − | − |
| N184D-N200H | − | − | |
| N184D-N200Q | − | − | |
| N184D-N200S | − | − | |
| N184E-N200D | − | − | |
| N184G-N200D | − | − | |
| N184T-N200T | − | − |
a
+, ≥ 55%; −, ≤10% (relative to wild-type PrP).