FIG. 5.

The Bro1 domain of AIP1 binds to Z and NP. (A) Schematic representation of wild-type AIP1 and the constructs encoding the AIP1 domains used in this study. The numbers indicate the positions of the domains within wild-type AIP1. Each construct was fused with a FLAG tag at its N terminus. Bro, Bro1 domain; V, “V” domain; PRR, proline-rich region. (B and C) pC-MopNP-HA (B) or pC-MopZ-HA (C) was cotransfected with pC-FLAG-AIP1_Bro, pC-FLAG-AIP1_V, or pC-FLAG-AIP1_PRR into 293T cells. After 48 h, proteins were precipitated with Dynabeads/anti-HA antibody complexes and analyzed by Western blotting with anti-HA and anti-FLAG antibodies.