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. 2011 Jan 26;85(7):3498–3506. doi: 10.1128/JVI.02586-10

FIG. 5.

FIG. 5.

Hybrids formed between nonpermissive MDTF and BHKPiT1 cells are susceptible to GALV infection. Hygromycin-resistant MDTF (MDTF-hygro) cells were generated by transducing MDTF cells with retroviral vector pLHCX, and BHKPiT1 and MDTFPiT1 cells were constructed with pLNSX-PiT1, which confers G418 resistance. The MDTF/BHKPiT1 hybrid was produced by coselection with hygromycin and G418 after fusion of MDTF-hygro with BHKPiT1 cells. MDTF-hygro, BHKPiT1, MDTF/BHKPiT1 hybrid, and MDTFPiT1 cells were exposed to GALV-enveloped retroviral vectors expressing nuclear localized β-galactosidase. Micrographs were taken with a 40× objective on a phase-contrast microscope.