FIG. 4.

Spt2 controls the histone H3 occupancy at the SRG1-SER3 locus. (A) Diagram showing the SRG1-SER3 regions analyzed by histone H3 ChIP. (B, C, and D) Spt2 is required to maintain normal histone H3 level at the SRG1 3′-end region corresponding to the SER3 promoter. Histone H3 ChIP assays were conducted using chromatin extracted from wild-type (WT) (YAN1034) and spt2Δ (YAN1035) strains with or without the pGAL1-SRG1 construct. For each strain, the value shown represents the ratio of the percent immunoprecipitation (%IP) at the indicated region to the %IP at NoORF. The values shown represent the average results with standard errors from three to six independent experiments.