TABLE 1.
Genotypes of the yeast strains used in this study
| Strain | Relevant genotype | Referencea |
|---|---|---|
| Wild type | ||
| YPH499 | MATaura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1 | 44 |
| YPH501 | MATa/α ura3-52/ura3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochre trp1-Δ63/trp1-Δ63 his3-Δ200/his3-Δ200 leu2-Δ1/leu2-Δ1 | 44 |
| cdc13-1 mutants | 14 | |
| TXJ002 | YPH501 EST1/est1Δ::TRP1 KU70/ku70Δ::HIS3/CEN pRS316-EST1/CEN pRS315-EST1 | |
| TXJ003 | YPH501 EST1/est1Δ::TRP1 KU80/ku80Δ::HIS3/CEN pRS316-EST1/CEN pRS315 | |
| TXJ004 | YPH501 EST1/est1Δ::TRP1 KU80/ku80Δ::HIS3/CEN pRS316-EST1/CEN pRS315-EST1 | |
| TXJ005 | YPH501 CDC13/cdc13Δ::ADE2 EST1/est1Δ::TRP1 KU70/ku70Δ::HIS3/CEN pRS316-EST1/CEN pRS415-CDC13-EST2 | |
| TXJ006 | YPH501 CDC13/cdc13Δ::ADE2 EST1/est1Δ::TRP1 KU80/ku80Δ::HIS3/CEN pRS316-EST1/CEN pRS415-CDC13-EST2 | |
| TXJ007 | YPH501 CDC13/cdc13Δ::ADE2 EST1/est1Δ::TRP1 KU80/ku80Δ::HIS3/CEN pRS316-CDC13-EST2/CEN pRS315-ku80-135i | |
| TXJ008 | YPH499 ku70Δ::HIS3 | |
| TXJ009 | YPH499 ku80Δ::HIS3 | |
| TXJ010 | YPH499 ku80Δ::HIS3 est1Δ::TRP1/CEN pRS316-EST1/CEN pRS315-ku80-135i | |
| TXJ011 | YPH499 ku80Δ::HIS3/CEN pRS315-ku80-135i | |
| TXJ012 | YPH499 ku70Δ::HIS3 est1Δ::TRP1 exo1Δ::ADE2/CEN pRS316-EST1 | |
| TXJ013 | YPH499 ku80Δ::HIS3 est2Δ::TRP1/CEN pRS316-EST2 | |
| TXJ014 | YPH501 ku80Δ::HIS3 est1Δ::TRP1 cdc13Δ::ADE2/CEN pRS316-EST1/CEN pRS317-CDC13-EST2/CEN pRS315-EST1 | |
| TXJ015 | YPH501 ku80Δ::HIS3 est1Δ::TRP1 cdc13Δ::ADE2/CEN pRS316-EST1/CEN pRS317-CDC13-EST2/CEN pRS315-est1-D514A | |
| TXJ016 | YPH499 ku80Δ::HIS3 est1Δ::TRP1 cdc13Δ::ADE2/CEN pRS316-EST1/CEN pRS317-CDC13-EST2/CEN pRS317-est1-60 | |
| TXJ017 | YPH501 EST1/est1Δ::TRP1/CEN pRS316-EST1 | |
| TXJ018 | YPH501 EST1/est1Δ::TRP1/CEN pRS316-est1-D514A | |
| TXJ019 | YPH501 EST1/est1Δ::TRP1 RAD50/rad50Δ::HIS3/CEN pRS316-EST1 | |
| TXJ020 | YPH501 EST1/est1Δ::TRP1 RAD50/rad50Δ::HIS3/CEN pRS316-est1-D514A | |
| TXJ021 | YPH501 EST1/est1Δ::TRP1 RAD51/rad51Δ::HIS3/CEN pRS316-EST1 | |
| TXJ022 | YPH501 EST1/est1Δ::TRP1 RAD51/rad51Δ::HIS3/CEN pRS316-est1-D514A |
a
Unless otherwise indicated with a reference number, all strains were constructed in this study.