TABLE 3.
Hemodynamic analysis before and after isoproterenol infusiona
| Parameter | Baseline value for: |
P(*) | Value after isoproterenol infusion (5 ng/kg/min) in control group (n = 7) | P(#) | Value after isoproterenol infusion (5 ng/kg/min) in F/F;cre group (n = 7) | P(#) | P(*) | |
|---|---|---|---|---|---|---|---|---|
| Control mice (n = 7) | F/F;cre mice (n = 7) | |||||||
| Age (days) | 74.57 ± 7.43 | 75.71 ± 0.48 | ||||||
| HW/BW (mg/g) | 4.58 ± 0.27 | 5.10 ± 0.36 | 0.011 | |||||
| HR (bpm) | 430 ± 41.83 | 446 ± 47.69 | 0.521 | 484.33 ± 37.32 | 0.000 | 481.64 ± 47.91 | 0.008 | 0.916 |
| ESP (mm Hg) | 71.88 ± 11.03 | 61.21 ± 8.11 | 0.061 | 88.93 ± 6.83 | 0.004 | 79.22 ± 8.51 | 0.001 | 0.037 |
| dP/dtmax (mm Hg/s) | 5,299 ± 1,892 | 3,698 ± 981 | 0.070 | 8,342 ± 1,971 | 0.000 | 6,393 ± 1,227 | 0.000 | 0.046 |
| dP/dtmax/IP (s−1) | 117.27 ± 26.06 | 103.96 ± 16.99 | 0.280 | 146.49 ± 31.86 | 0.003 | 127.00 ± 20.76 | 0.003 | 0.200 |
| EDP (mmHg) | 3.26 ± 0.88 | 3.52 ± 1.43 | 0.691 | 3.07 ± 0.86 | 0.408 | 3.39 ± 1.36 | 0.643 | 0.605 |
| dP/dtmin (mm Hg/s) | −4,925 ± 1,870 | −3,687 ± 1,323 | 0.178 | −6,974 ± 1,886 | 0.015 | −5,966 ± 1,685 | 0.004 | 0.403 |
| Adj. PMX (mW/μl2) | 43.91 ± 26.34 | 32.92 ± 15.74 | 0.362 | 65.79 ± 29.79 | 0.001 | 55.02 ± 12.10 | 0.002 | 0.393 |
| EF (%) | 43.61 ± 15.31 | 41.40 ± 10.76 | 0.760 | 50.56 ± 15.77 | 0.002 | 53.21 ± 5.71 | 0.011 | 0.682 |
| Cardiac output (μl/min) | 8,458 ± 3,149 | 8,397 ± 2,495 | 0.969 | 10,862 ± 3,730 | 0.001 | 11,561 ± 2,639 | 0.007 | 0.693 |
| τG (ms) | 16.18 ± 6.33 | 15.10 ± 5.10 | 0.730 | 13.84 ± 4.89 | 0.089 | 12.49 ± 3.77 | 0.171 | 0.573 |
The control group consists of 4 F/F;− mice and 3 +/+;cre mice. Values are means ± standard deviations. P(*), comparisons for significance between control and F/F;cre groups were made by two-tailed unpaired t test. P(#), comparisons for significance within a given baseline and isoproterenol-treated group were made by two-tailed paired t test. The P values that are below the 0.05 level of significance are shown in bold. HW/BW, heart weight to body weight ratio; HR, heart rate; ESP, end-systolic pressure; dP/dtmax, peak rate of pressure increment; dP/dtmax/IP, peak rate of pressure increment normalized by instantaneous developed pressure (IP); EDP, end-diastolic pressure; dP/dtmin, peak rate of pressure decrement; Adj. PMX, maximum power adjusted by preload; EF, ejection fraction; τG, relaxation time constant calculated by the Glantz equation.