FIG. 2.

Focal spread of RDV strain O in the presence of virus-neutralizing antibodies. VCM were inoculated for 2 h with RDV strain O at an MOI of 0.0002 and cultured for 10 days in the presence of virus-neutralizing antibodies, which prevent infection by non-cell-associated virus particles. Viruses were labeled with Pns10-specific IgG conjugated with rhodamine (red; RDV Pns10) and viral-particle-specific IgG conjugated to Alexa 488 (green; RDV particle). Immunostaining was visualized by confocal fluorescence microscopy. “Merge” is a merged view of “RDV Pns10” and “RDV particle.” “Mock” is a view of cells inoculated with healthy plant materials. Scale bar, 30 μm.