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VP11/12 is not required for infection-induced phosphorylation of the mTORC1 substrates S6K and 4E-BP1. HEL fibroblasts were infected with HSV-1 KOS37, ΔUL46galK, ΔUL46, and RUL46. At 20 h postinfection, lysates were collected and analyzed for phosphorylation of S6K at T389 (anti-pT389), total S6K, and total 4E-BP1 by Western blotting.
