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VP11/12 is tyrosine phosphorylated and promotes SFK activation in infected HEL fibroblasts. HEL fibroblasts were infected with HSV-1 KOS37, ΔUL46galK, ΔUL46, and RUL46. Cell lysates were collected at 21 h postinfection and analyzed by Western blotting. Western blot detection of SFKs phosphorylated at the activating tyrosine (the equivalent of phospho-Y416 of Src, anti-active SFK), phosphotyrosine (anti-pTyr), VP16, and β-actin.
