FIG. 3.

AG879 and A9 inhibit the later stages of the influenza virus life cycle. (A) Time course analysis of the AG879 and A9 inhibitory effects on influenza A virus replication. A549 cells were infected with A/WSN at an MOI of 1 and, at different time points, treated with DMSO or the respective inhibitor. Virus titer at 9 hpi was determined by plaque assay. (B) Effects of AG879 and A9 on virus attachment. A549 cells were preincubated with DMSO or chemicals for 30 min and infected with A/WSN (MOI of 2) at 4°C for 2 h. After cells were washed 6 times with PBS, cell lysates were prepared by rapid freeze-thaw. Virus titer was determined by plaque assay. Results shown are the averages from at least three independent experiments. Error bars indicate SD.