FIG. 3.

Nef-mediated downregulation of HLA-A31 by nonintegrated virus. Analysis of infected GFP-positive cells (gate R2) for cell surface HLA-A31 expression. Representative results for wt-integrase-bearing virus (a). Virus with wild-type nef downregulates HLA-A31 to a greater degree than Δnef virus. The results are overlaid in the histogram; wt virus is displayed in white and Δnef virus in shaded gray. A similar pattern of results is seen for virus bearing the integrase-deficient D116N mutation (b) or with virus bearing wt integrase but in the presence of inhibitory concentrations of raltegravir (c). The bar chart shows combined results of 3 independent experiments with duplicate infections with wt or defective D116N integrase (IN) with raltegravir (RAL) (d). Geometric means of fluorescence for each receptor are expressed relative to the level after infection by the Δnef virus or that for a no-drug control (100%). Error bars indicate standard deviations (SD).