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. 2011 Jan 5;85(6):2828–2836. doi: 10.1128/JVI.01854-10

FIG. 5.

FIG. 5.

Nef-mediated downregulation of HLA-ABC in activated primary CD4+ T cells by nonintegrated virus. Analysis of infected live cells for cell surface HLA-ABC expression. Representative histogram plot for wt-integrase-bearing virus (white) versus Δnef virus (shaded gray) (a). Virus with wt nef downregulates HLA-ABC to a greater extent than Δnef virus. Representative histogram plot for wt-integrase-bearing virus (white) versus Δnef virus (shaded gray) in the presence of 2 μM raltegravir (b). Virus with wild-type nef downregulates HLA-ABC to a greater extent than Δnef virus. Combined results of two independent experiments of duplicate infections with wt integrase virus in the absence of drug (wt IN) or in the presence of 2 μM raltegravir (wt IN + RAL) (c). Geometric means of fluorescence are expressed relative to the level after infection by the Δnef virus (100%). Error bars indicate standard deviations (SD). Values for wt and Δnef viruses were tested for statistical significance using an unpaired two-tailed t test (*; P < 0.05; **, P < 0.01).