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. 2011 Jan 5;85(6):2666–2685. doi: 10.1128/JVI.01532-10

FIG. 4.

FIG. 4.

Quantitative real-time PCR demonstrating KSHV infection in HMVEC-d cells. (A) BCBL-1 cells were treated with 200 μM neomycin or 20 ng/ml TPA for 3 days, virus was purified from the supernatant, DNA was extracted, and ORF73 DNA copy numbers were determined. (B) HMVEC-d cells were infected with KSHV (10 DNA copies/cell) purified from neomycin-treated BCBL-1 cells for the indicated time points, DNA was extracted, and ORF73 DNA copy numbers were determined. (C) HMVEC-d cells were infected for 2, 8, and 24 h with KSHV (10 DNA copies/cell) purified from neomycin-treated BCBL-1 cells. Total RNA was extracted, converted to cDNA, and used for measurement of ORF73 and ORF50 gene expression by quantitative real-time PCR and normalized to that of tubulin. ***, P < 0.0001.