FIG. 1.

siRNA-RNAi knockdown of EBOV genes. (A and B) HEK293 and Vero cells were transfected with plasmids encoding EBOV nucleoprotein (pNP) or glycoprotein (pGP) alone, together with the nontargeting siRNA, or with siRNAs targeting the viral mRNAs. Cell extracts were analyzed by SDS-PAGE, proteins were detected by Western blotting with antibody to either NP or GP, and expression levels were normalized against β-actin expression. The experiments were performed in triplicate. (C) Vero cells were transfected with siNPs or siGPs and, after 4 h, infected with EBOV at an MOI of 0.2. Results are presented as percentages of EBOV-infected cells in the presence of nontargeting siRNA. Values are averages ± standard deviations (SD) from at least two independent experiments.