FIG. 3.

Interactions of EBOV VP30 and VP35 with Dicer, TRBP, and PACT. FLAG-tagged VP30 and VP35 were coexpressed in HEK293 cells in the presence of pGFP together with siGFP or in the absence of pGFP and siGFP. An untransfected sample and empty vector (pVR1012) were used as negative controls. Immunoprecipitates were analyzed by SDS-PAGE followed by Western blotting using an anti-FLAG antibody (α-FLAG). Immunoprecipitation (IP) was carried out with anti-Dicer (A), anti-TRBP (B), and anti-PACT (C) antibodies. pluc-F was used as a negative control. Endogenous levels of Dicer, TRBP, and PACT were detected by probing with antibodies to Dicer, TRBP, and PACT. Input lanes contain 10% of the total cell lysates (D). This experiment was performed three times.