FIG. 5.

Insertion of a non-G-rich transcript disrupts OriLyt function. (A) Wild-type or mutant pBluescript-OriLyt or vector control plasmids were transected into ZKO-293 cells with or without the BZLF1 gene. Plasmids were analyzed by Southern blotting before (data not shown) or after (as indicated) transfection and lytic induction. Plasmids recovered from cells were digested with or without DpnI enzyme (as indicated). One representative experiment is shown. (B) Relative plasmid amounts were quantified using quantitative Southern blotting. Results were calculated as the DpnI-digested signal divided by the input plasmid signal and normalized to the value obtained for wild-type OriLyt. Data averages from three independent experiments are shown, with error bars representing the standard deviations. Statistical significance was determined using a two-tailed, unpaired t test.