Skip to main content
. 2010 Dec 29;85(6):3010–3014. doi: 10.1128/JVI.01241-10

FIG. 1.

FIG. 1.

Expression and characterization of HA-HuFc fusion proteins. (A) Baculovirus-expressed HA-HuFc was present in cell extracts (track 1) and in supernatant (track 2) and following purification (track 3). (B) Western blot analysis performed with an anti-Hu Ig conjugate showed intermediates present in cell extracts but no evidence of breakdown in the supernatant or following purification. (Tracks are as described for panel A.) (C) Velocity gradient analysis of purified HA-HuFc showed a distribution of solution molecular masses estimated to range from dimers (∼200 kDa) to hexamers (∼600 kDa). No aggregate was present in the heavier fractions. Numbers to the left in panels A and C represent molecular mass markers, with values in kilodaltons. The numbers at the top of panel C represent solution molecular mass makers taken from a parallel gradient analysis, with values in kilodaltons. Panels A and C show stained 10% SDS-PAGE gels, and panel B shows chemiluminescence. Data shown are representative of the H3 subtype and are typical of other HA subtypes.