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. 2011 Jan 5;85(6):2599–2610. doi: 10.1128/JVI.02213-10

FIG. 1.

FIG. 1.

RelA controls basal expression of ISGs via regulation of autocrine IFN-β. (A) List of the top 10 most-disrupted (downregulated) canonical pathways in unstimulated rela−/− MEFs as generated by Ingenuity Pathways Analysis. Fisher's exact test was used to calculate a P value determining the probability that the downregulation of genes in the canonical pathway is explained by chance alone. (B) Wild-type (WT) MEFs were stimulated with IFN-β (200 U/ml for 6 h). All genes induced at a ≥2-fold level were subsequently evaluated for basal expression in stat1+/+ and stat1−/− MEFs. The top 50 genes that were most highly induced by IFN-β in WT MEFs (left) while simultaneously displaying the lowest basal levels in stat1−/− MEFs (right) were defined as the autocrine IFN-β signature. Expression in stat1+/+ MEFs was normalized to one (20; yellow). (C) Analysis of the autocrine IFN-β signature in unstimulated rela+/+ (normalized to yellow) and rela−/− MEFs. (D) Analysis of the autocrine IFN-β signature in unstimulated rela+/+ (normalized to yellow) and rela−/− MEFs on an ifnar1−/− background. Heat bars on the left represent relative expression levels on a log2 scale.