Figure 3.

Analysis of Polyfunctional CD4⁺ T cell Responses. A, interferon (IFN)–γ, interleukin (IL)–2, tumor necrosis factor (TNF)–α, and IL-4 responses to Env peptides were assessed 2 weeks after receipt of the final protein boost by intracellular cytokine staining. Pie charts show the percentage of Env-specific cells responding with secretion of 1, 2, 3, or 4 cytokines averaged for individuals receiving DNA priming (groups 1–4; left) or for individuals receiving protein alone (group 5; right). Arcs show the percentage of cells producing each of the 4 cytokines. The color keys for the pie slices and arcs are shown. B, Specific cytokine secretion patterns are shown for those cells expressing 1 cytokine (left), 2 cytokines (middle), or 3 cytokines (right) following in vitro peptide stimulation. Red dots indicate responses from individuals primed with DNA (groups 1–4); blue dots indicate responses from individuals receiving protein alone (group 5).