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Measurement of Neutralizing Antibodies Against CD4i Epitopes. Human immunodeficiency virus (HIV)–2 7312A/V434M is a molecularly-cloned HIV-2 isolate that is susceptible to neutralization by CD4i antibodies in the presence of subinhibitory concentrations of sCD4. In this assay, SF162 or HIV-2 were incubated in the absence (left) or presence (right) of 0.5 μg/mL sCD4, and neutralization of virus assayed using TZM-bl luciferase reporter cells.
