Figure 4.

IFN-γ production in response to controls and to 5 recombinant Burkholderia pseudomallei proteins of healthy individuals and recovered melioidosis patients. Whole-blood samples of 20 healthy individuals and 30 recovered melioidosis patients were cocultured with controls; medium, phytohemagglutinin (PHA), and Franciscella tularensis FT1823 (unrelated protein) (A), and 5 selected B. pseudomallei proteins (B) for 48 h. The cultured supernatants were assayed for IFN-γ by means of ELISA. The distribution of IFN-γ levels was plotted and compared for healthy individuals vs recovered patients with use of the Mann-Whitney t test. One asterisk indicates P < .05. ns, nonsignificant.