Table 1.
Top Burkholderia pseudomallei Protein Antigens Identified by Microarray Chips Probed with Plasma Samples from Recovered Melioidosis Patients and/or Seropositive Healthy Individuals
| Locus tag | Protein name | Classification | Predicted function associated | Recovered | Healthy | |
| Top proteins of both healthy control participants and recovered melioidosis patients | ||||||
| BPSL2697 | Chaperonin GroEL | Chaperones | Pathogenicity/adaption/chaperones | 235,714 (18,662) | 46,831 (9286) | |
| BPSS1532 | Cell invasion protein (bipB) | Pathogenicity island-related functions | Pathogenicity/adaption/chaperones | 147,514 (19,384) | 34,004 (6196) | |
| BPSS1512 | Type VI secretion protein, TssM | Hypothetical protein | Unknown/hypothetical | 185,219 (20,903) | 42,267 (8240) | |
| BPSS0477 | 60 kDa chaperonin | Chaperones | Pathogenicity/adaption/chaperones | 168,271 (18,095) | 30,034 (6728) | |
| BPSL3319 | Flagellin | Chemotaxis and mobility | Pathogenicity/adaption/chaperones | 140,395 (17,474) | 48,672 (7493) | |
| BPSL2765 | Putative OmpA family lipoprotein | Membrane/exported/lipoproteins | Surfacea | 74,103 (11,987) | 20,976 (3272) | |
| BPSL2520 | Putative exported protein BPSL2520 | Membrane/exported/lipoproteins | Surfacea | 71,886 (14,942) | 8,912 (3333) | |
| BPSS1492 | Hypothetical protein Bim A | Hypothetical protein | Unknown/hypothetical | 67,895 (15,980) | 28,538 (6786) | |
| BPSL0999 | Putative OmpA family lipoprotein | Inner membrane | Surfacea | 66,679 (14,547) | 14,915 (4195) | |
| BPSL1901 | Putative membrane protein | Inner membrane | Surfacea | 46,354 (9461) | 31,068 (6991) | |
| BPSL1445 | Putative lipoprotein | Membrane/exported/lipoproteins | Surfacea | 34,941 (9806) | 10,154 (4485) | |
| BPSL3398 | ATP synthase alpha chain | ATP-proton motive force | Energy metabolismb | 27,222 (6481) | 9711 (1887) | |
| BPSS1974 | Putative lipoprotein | Membrane/exported/lipoproteins | Surfacea | 24,870 (5952) | 26,429 (5590) | |
| Top proteins of recovered melioidosis patients | ||||||
| BPSL0280 | Flagellar hook-associated protein | Chemotaxis and mobility | Pathogenicity/adaption/chaperones | 75,283 (13,077) | 1130 (521) | |
| BPSS1588 | Putative exported protein BPSS1588 | Membrane/exported/lipoproteins | Surfacea | 46,124 (11,096) | 367 (218) | |
| BPSL2522 | Outer membrane protein A (OmpA) precursor | Outer membrane | Surfacea | 40,296 (10,380) | 1685 (371) | |
| BPSS1599 | Type IV pilus biosynthesis protein (pilO) | Outer membrane | Surfacea | 39,847 (7759) | 2995 (906) | |
| BPSL2096 | Putative hydroperoxide reductase | Detoxification | Pathogenicity/adaption/chaperones | 31,387 (4269) | 2727 (837) | |
| BPSL3222 | 50S ribosomal protein L7/L12 | Ribosomal proteins—synthesis, modification | Information transferc | 29,224 (7566) | 6291 (2034) | |
| BPSS2141 | Periplasmic oligopeptide-binding protein precursor (OppA) | Carbohydrates, organic acids, and alcohols | Surfacea | 23,927 (5596) | 2133 (1321) | |
| Top protein of healthy controls | ||||||
| BPSL1465 | Peptidase | Membrane/exported/lipoproteins | Surfacea | 19,073 (2819) | 13,898 (2290) | |
| BPSL1661 | Putative hemolysin-related protein | Membrane/exported/lipoproteins | Surfacea | 10,255 (3185) | 16,491 (4924) | |
| BPSL1902 | Putative membrane protein | Inner membrane | Surfacea | 15,381 (3964) | 11,748 (2729) | |
| BPSL2063 | Putative membrane protein | Inner membrane | Surfacea | 23,599 (5191) | 22,611 (4560) | |
| BPSS0734 | Outer membrane efflux protein | Outer membrane | Surfacea | 7707 (2790) | 11,574 (3924) | |
| BPSS1434 | Putative membrane-anchored cell surface protein | Inner membrane | Surfacea | 10,840 (2676) | 19,734 (3559) | |
| BPSS2053 | Putative cell surface protein | Surface structures | Surfacea | 18,748 (4652) | 28,006 (5556) |
NOTE. Protein locus tag, name, and classification and function of B. pseudomallei proteins that were predicted on the basis of the Artemis database on the Sanger website. The mean signal intensity and standard error of the mean (SE) of individual proteins from 108 healthy control individuals and 72 recovered melioidosis patients were calculated using Excel software (Microsoft). Data are mean (SE) unless otherwise specified.
Surface consisted of outer membrane, inner membrane, and secreted and surface structures.
Information transfer consisted of transcription/translation and DNA/RNA modification.
Energy metabolism consisted of glycolysis, electrontransport, etc.