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. 1995 Mar 25;23(6):1060–1067. doi: 10.1093/nar/23.6.1060

Coding sequence composition flanking either signal element alters V(D)J recombination efficiency.

N V Boubnov 1, Z P Wills 1, D T Weaver 1
PMCID: PMC306805  PMID: 7731794

Abstract

Lymphoid V(D)J rearrangement is targeted by recombination signal sequences (RSS) bordering V, D or J exons. We demonstrate that the DNA composition of flanking coding positions, particularly poly(A) or poly(T) stretches at one or both RSS, diminishes V(D)J recombination up to 100-fold. Positionally correct cleavages occur in the inhibited reactions, since the junctions formed show the same frequency of precision as uninhibited reactions. Open/shut cleavage/rejoining is not increased at a normal RSS in substrates containing inhibitory A/T homopolymers versus random sequence at a second RSS. Thus recombinase action at both cleavage sites is severely disrupted by modified coding sequences.

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Selected References

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