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Titration of response to NY-ESO-1 peptide. Soluble interferon gamma (IFN-γ) levels were measured in overnight cocultures of 105 cells from samples of infused transduced T cells from patients with (A) melanoma and (B) synovial cell sarcoma with 105 T2 cells that had been pulsed with the NY-ESO-1:157-165 peptide. Responses to the negative control gp100:154-162 peptide were all less than 100 pg/mL of IFN-γ.
