Table 3.
Parameters used to assemble the individual and composite white spruce linkage maps.
Mapping parameters | Individual | maps | Composite map | ||
---|---|---|---|---|---|
Cross P | Cross D | ||||
♀80112 | ♂80109 | ♀77111 | ♂2388 | ||
Available markers a | 600 | 671 | 521 | 508 | 1,578 b |
Distorted markers c | 10 | 12 | 15 | 19 | 33 |
Markers without segregation distortion | 590 | 659 | 506 | 489 | 1545 |
Assigned markers | 580 | 650 | 486 | 470 | 1,342 |
AFLP loci | 256 | 289 | - | - | 470 |
SSR loci | 9 | 10 | - | - | 12 |
Gene loci | |||||
ESTPs | 22 | 23 | 20 | 20 | 33 |
SNPs | 293 | 328 | 466 | 450 | 827 d |
Total | 315 | 351 | 486 | 470 | 860 |
Positioned marker loci (%) | 549 (94.7) | 570 (87.7) | 471 (96.9) | 454 (96.6) | 1,301 (96.9) |
AFLP loci | 239 | 235 | - | - | 453 |
SSR loci | 8 | 10 | - | - | 12 |
Gene loci | |||||
ESTPs | 19 | 19 | 20 | 20 | 33 |
SNPs | 283 e | 306 f | 451 | 434 | 803 g |
Total | 302 | 325 | 471 | 454 | 836 |
Major linkage groups (n > 10 markers) | 12 (4 h) | 12 (1 h) | 12 | 12 | 12 |
Minor linkage groups (3 ≤ n ≤ 10 markers) | 1 | 0 | 0 | 0 | 0 |
Unlinked markers | 10 | 9 | 20 | 19 | 58 |
Map length GF, cM (Kosambi) | 2,163.6 | 2,276.1 | 2,055.6 | 1,700.1 | 2,086.8 |
Average map density, cM (Kosambi) | 3.9 | 4.0 | 4.4 | 3.7 | 1.6 |
Average size for major linkage groups, cM (Kosambi) | 143.3 | 162.6 | 171.3 | 141.7 | 173.9 |
a For individual linkage maps, only markers segregating 1:1 or 1:1:1:1 were used.
b 256 markers were shared between the two crosses.
c Bonferroni correction: P ≤ 0.01/number of loci.
d 373 gene loci from Arborea PgLM0 SNP array, 339 from Arborea PgLM1 array, and 115 from Treenomix array.
e 215 gene loci from Arborea PgLM0 SNP array, and 68 from Treenomix array.
f 239 gene loci from Arborea PgLM0 SNP array, and 67 from Treenomix array.
g 370 gene loci from Arborea PgLM0 SNP array, 325 from Arborea PgLM1 array, and 108 from Treenomix array.
h Number of linkage groups composed of 2 subgroups having more than 10 markers.