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. 2011 Mar 14;10:60. doi: 10.1186/1475-2875-10-60

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Copyright ©2011 Arévalo-Pinzón et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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PvRON2 expression and apical localization. A. Anti-PvRON2 rabbit polyclonal antibodies detected two bands at around ~220 and ~185 kDa in parasite lysate by Western blot. Lane 1: pre-immune serum. Lane 2: hyper-immune serum. B. P. vivax schizonts incubated with anti-PvRON2 polyclonal antibodies and revealed with FITC-labelled anti-rabbit IgG (green). Parasite nuclei were stained with DAPI (blue). C. Co-localization study: schizonts were simultaneously incubated with anti-PvRON2 and anti-PvAMA-1 (top) or anti-PvRhopH3 (bottom) and detected with FITC-labelled anti-rabbit and with rhodamine-labelled anti-mouse. Arrows indicate the typical dotted pattern displayed by apical organelles.