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. 2011 Mar 30;6(3):e18085. doi: 10.1371/journal.pone.0018085

Figure 2. Influence of CR on tumor growth, body weight, and plasma biomarkers.

Figure 2

Tumors were implanted i.c. on day 0. All tumor-bearing mice were fed ad libitum (AL) for the first 48 hrs after tumor implantation and were then randomly assigned to one of two diet groups that received rodent chow in either unrestricted (AL; n = 4) or calorically restricted (CR; n = 5) amounts. The two groups were matched for body weight (∼28.0 g) prior to the initiation of CR. The CR group received a feeding regimen of 30% less or 70% of food of AL mice consume daily. All mice were sacrificed 14–15 days after tumor implantation, as described above in the Materials and Methods section. Final weights of the mice were obtained immediately prior to sacrifice (B). The asterisk in indicate that the tumor growth (A), body weight (B), and glucose level (C) is significantly reduced and ketone level (D) is significantly increased in the CR group compared with the AL group at * P<0.05, ** P<0.001 (Student t-test).