Figure 4. Peptide-based caspase inhibitor impairs the transition from M to G₁ phases through inhibiting APC/C.

A) HeLa.S-Fucci cells were cultured in the presence of 0.6% DMSO or 200 µM Z-Asp-CH₂-DCB for 48 hours, and were observed with the time-lapse fluorescence microscope at 30-minute intervals (see Videos S6 and S7). The morphology of cells and the color of cell nuclei at 0 and 48 hours are represented. Ten mitotic cells with a round shape and a green color were randomly picked up from cells treated with DMSO (#1 to #10) and Z-Asp-CH₂-DCB (#11 to #20). B) The merged images of the mitotic cells (#11 to #20 in A) at 5 hours after treatment with Z-Asp-CH₂-DCB were shown by phase contrast, monomeric Azami-Green 1 (mAG1), and monomeric Kusabira-Orange 2 (mKO2) images, respectively.