Table 3. Cost Estimates for Pooled Sequencing Projects.

			750 Kb Capture Project				3 Mb Capture Project
	Library Prep			Total Project				Total Project
	Cost ($1000)		Sequencing	Cost ($1000)		Cost	Sequencing	Cost ($1000)		Cost
Number of			Cost ($1000)			Difference	Cost ($1000)			Difference
Samples	Single Plex	Pooled		Single Plex	Pooled			Single Plex	Pooled
400	110	5.5	2.7	112.7	8.2		10.7	120.7	16.2
4000	1100	55	26.8	1126.8	81.8	13.8	107	1207	162	7.5
10000	2750	137.5	66.9	2816.9	204.4		267.6	3017.6	405.1

Cost estimates based on a 100 bp HiSeq paired-end run with Illumina's published reagent costs ($11,150 per flowcell) and an average throughput of 200 Gb per run. Sample preparation includes $75 per samples for library prep and $200 per sample for solution based capture. All calculations for pooled sequencing assume 20 individuals per pool.