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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Lab Invest. 2010 Nov 22;91(4):488–498. doi: 10.1038/labinvest.2010.188

Figure 4.

Figure 4

Endothelial Lipase is readily detected in urine of diabetic nephropathy patients. (a) Immunoblot analysis for Endothelial Lipase (57KDa) (EL) and Albumin (67KDa) in urine samples of diabetic (lanes A, H, I and K) and non-diabetic controls (lanes E, F, and J). High levels of EL (green) are detected in urine samples of two diabetic patients (H and I). Controls show no EL excretion. Albumin (red) was detected in all samples. The lower panel shows distinct migration of EL and Albumin. (b) EL antibody does not cross react with albumin. Dot blot pattern of EL and Albumin immunostaining to different amounts of purified human albumin protein shows no immunostaining with EL antibody further confirming that EL detection is specific.