Skip to main content
. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: J Hepatol. 2010 Dec 13;55(2):289–298. doi: 10.1016/j.jhep.2010.11.019

Figure 2. IL28B induces STAT1 and STAT2 phosphorylation and ISRE reporter activity.

Figure 2

OR6 cells (A) or JFH1-infected Huh7.5.1 cells (B) were treated with 100 ng/ml IL28B, 30 IU/ml IFNα or mock treatment for 30 min and the cell lysates were analyzed by Western blotting with the indicated antibodies. pISRE-luc encoding firefly luciferase under the control of the ISRE and pRL-TK expressing Renilla luciferase were transfected to Huh7.5.1 cells (C) or JFH1-infected Huh7.5.1 cells (D) for 48 hours and 100 ng/ml IL28B or 30 IU/ml IFNα were added to the cells for 6 hours. The firefly and Renilla luciferase activity was measured.