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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: J Hepatol. 2010 Dec 13;55(2):289–298. doi: 10.1016/j.jhep.2010.11.019

Figure 3. IL28B stimulates known ISGs.

Figure 3

Figure 3

(A) OR6 cells were incubated 10 ng/ml IL28B or 15 IU/ml IFNα or mock treatment for different times as indicated. Total RNA was isolated and reverse transcribed followed by real time PCR. OR6 cells (B) and JFH1-infected Huh7.5.1 cells (C) were incubated with 100 ng/ml IL28B for different times as indicated. Cell lysates were analyzed by immunoblotting with the indicated antibodies. (D) Huh 7.5.1 cells were incubated 100 ng/ml IL28A, IL28B, IL29 or mock treatment for different times as indicated. Total RNA was isolated and reverse transcribed followed by real time PCR.