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. Author manuscript; available in PMC: 2012 Mar 23.
Published in final edited form as: J Am Chem Soc. 2011 Feb 25;133(11):4046–4052. doi: 10.1021/ja110256c

Figure 3.

Figure 3

Competition between cis → trans thermal isomerization and binding of s11 to cis GFP: loop:s11 to form GFP: loop:s11•s11 starting from the initial photostationary mixture of refolded GFP: loop:s11 generated by light activating trans GFP: loop:s11 with 460 nm light (blue). The spectra following from blue to green are shown for every 1.2 minutes after mixing in s11 in the dark. The cartoon inset shows the kinetic model that describes the processes taking place, namely cis GFP: loop:s11 is partitioning between binding s11 to form GFP: loop:s11•s11 and reverting back to trans GFP: loop:s11. The initial concentrations of trans GFP: loop:s11 and s11 were 2 μM and 10 μM, respectively, and they were in pH 8 buffer at 18°C.