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. Author manuscript; available in PMC: 2012 Mar 23.
Published in final edited form as: J Am Chem Soc. 2011 Feb 25;133(11):4046–4052. doi: 10.1021/ja110256c

Figure 4.

Figure 4

Absorbance spectra of cis GFP: loop:s11 (yellow), trans GFP: loop:s11 (blue), and GFP: loop:s11•s11 (green). The absorbance spectra of GFP: loop:s11•s11 (green) and trans GFP: loop:s11 (blue) are reproduced from Figure 1B for comparison. The cis GFP: loop:s11 absorbance spectrum is derived from observing the changes in the photostationary state of GFP: loop:s11 with pH and photoactivation wavelength. These absorbance spectra are used to fit the data in Figures 2 and 3 to determine the concentrations of cis GFP: loop:s11, trans GFP: loop:s11 and GFP: loop:s11•s11.