Table III.
Apparent affinities of ATP, CTP, GTP, and dATP for WT CLC-1 and V634F
| Channels/mutants | Ko or Kc | |||
| ATP | CTP | GTP | dATP | |
| mM | mM | mM | mM | |
| Kc | ||||
| WT CLC-1 | 0.32 ± 0.04 (8) | 1.1. ± 0.2 (6) | 9.9 ± 1.0 (5) | 0.85 ± 0.14 (4) |
| V634F | 0.017 ± 0.005 (7) | 3.5 ± 0.4 (8) | 4.0 ± 0.6 (6) | 0.049 ± 0.013 (6) |
| Ω = 60 | Ω = 7.6 | Ω = 1.09 | ||
| Ko | ||||
| WT CLC-1 | 0.70 ± 0.10 (8) | 1.3. ± 0.3 (6) | 15.1 ± 1.9 (5) | 1.3 ± 0.2 (4) |
| V634F | 0.039 ± 0.013 (7) | 8.2 ± 1.2 (8) | 8.5 ± 1.6 (6) | 0.071 ± 0.021 (6) |
| Ω = 113 | Ω = 10 | Ω = 0.98 | ||
Calculated from the values of Poc at −40 mV. Means ± SEM are given. The numbers inside parentheses indicate the number of patches.