Skip to main content
. 2011 Jan 20;43(3):129–137. doi: 10.3858/emm.2011.43.3.014

Figure 3.

Figure 3

Induction of apoptosis by siNOX1/4 in 253J-BV cells. (A, B and C) 253J-BV cells were starved with 0.5% FBS medium for 12 h, and then cells were treated with DPI (5 µM), NAC (10 mM) for 48 h. (A) DPI or NAC induced cell death in 253J-BV cells. Cell morphology was visualised using an Olympus BX51 microscope at ×100 magnification. Bar, 100 µm. (B and C) DPI and NAC induced PARP cleavage (B) and DNA laddering (C) in 253J-BV cells. The detail apoptotic analysis is described in the Supplemental material and methods. (D) The levels of NOX mRNA transcripts were measured by semi-quantitative RT-PCR at 24 h after transfection with pSUPER-siNOX1/4 (1 µg). (E) Cell cycle arrest and PARP cleavage were induced by knockdown NOX1/4. Cells were transfected with pSUPER-siNOX1/4 (1 µg) and, after 42 h, the sub-G1 population was analysed using flow cytometry (left panel). PARP cleavage was also determined (right panel). Data indicate the means ± S.D. of three independent experiments (*P <0.01 vs pSUPER control vector).