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Enhanced gene transduction efficiency in m-ccdPA. Integrated copy numbers (A) and LCAT activity in the culture medium (B) after retroviral vector-mediated human lcat gene transduction were analyzed. Single round (b) and two rounds (a, c) of exposure to CGT_hLCATRV in the presence of 8 (a) or 500 µg/ml (b, c) of PS. Transductions with 8 µg/ml (a) and 500 µg/ml (b, c) of PS were performed overnight and one hour, respectively. Data are presented as the mean ± SD (n=3). *P<0.05.
