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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Protein Expr Purif. 2011 Feb 12;77(2):224–230. doi: 10.1016/j.pep.2011.02.004

Figure 5.

Figure 5

SDS-PAGE results for the purification of MBP-LBT-LR11TM-His6. Lanes: 1, protein marker; 2, elute of MBP-LBT-LR11TM-His6 from a Ni-NTA column; 3, thrombin cleavage of the protein sample in lane 2; 4 to 7, elutes of LBT-LR11TM-His6 from Ni-NTA columns with an elution buffer containing 0.2% DPC, 0.3% LDAO, 0.2% LMPG, and 0.2% DDM, respectively.