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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Protein Expr Purif. 2011 Feb 12;77(2):224–230. doi: 10.1016/j.pep.2011.02.004

Figure 8.

Figure 8

“In situ” thrombin cleavage of MBP-LBT-LR11TM-His6 at E. coli membrane surface. Lanes: 1, protein marker; 2, resuspension of MBP-LBT-LR11TM-His6 membrane fraction; 3, thrombin cleavage of the protein sample in lane 2; 4: flow-through of the sample in lane 3 from a Ni-NTA column; 5: elute of the sample in lane 3 from a Ni-NTA column.