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(a) Analysis of purified recombinant Amy1 on 12% SDS-PAGE; M, standard marker proteins; lane 1, lane 2, protein profile after ion exchange on Q-Sepharose column; lane 3, purified recombinant amylase after gel filtration on Sephacryl S-200HR column. (b) Detection of amylase activity on non-denaturing PAGE.
