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Distribution of cholesterol during sperm maturation in murine epididymis. (a) Sperm collected from caput epididymis reveal filipin signal on the whole head. After epididymal transit (cauda epididymis), the signal is not detected in the postacrosomal region (PA). Scale bar = 5 μm. (b) Densitometric analysis shows a significant decrease of filipin signal at the postacrosomal region. AA, apical acrosome; ES, equatorial segment; PA, postacrosomal.
