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Orientation of Na+ K+-ATPase in proteoliposomes measured by the sidedness assay (n = 3). Proteoliposomes were created by nitrogen filtration, incubated ± ouabain ± detergent and then function (PO4 production from ATP) was measured and used to calculate sidedness [33] to determine the relative amount of Na+ K+-ATPase that was facing inside out, right side out, or was not incorporated. All three orientations of enzyme were statistically different from each other (P < .05).
