Fig. 2.

Agarose gel electrophoresis analysis of LDH isoforms. LDH isoforms from Cyto and MI in control primary breast cell line (HMEC 184), and breast cancer cell lines (MCF-7, MDA-MB-231) were separated by agarose gel electrophoresis. Total protein of 12 μg was loaded in wells (1, 2, 3, 4, 5, and 6), and 48 μg in wells (7, 8, and 9). The MCF-7 cell line expressed mainly LDHB, an LDH isoform found in oxidative cell lines. The HMEC 184 and MDA-MB-231 cell lines expressed mainly LDHA, an LDH isoform found in glycolytic cell lines. Data are from 2 different experiments.