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. 2010 Dec 21;43(5):237–254. doi: 10.1152/physiolgenomics.00193.2010

Fig. 1.

Fig. 1.

Gene validation by RT-qPCR of selected genes. Based on microarray gene expression data 4 genes previously known to be selectively expressed in 1 segment (A) were chosen and mRNA expression assessed by RT-qPCR. Significant enrichment (log ratio >1) was found for colipase in gastric epithelial cells, intestinal alkaline phosphatase in proximal small bowel, matrilysin in distal small bowel, and mucosal pentraxin in colonic epithelial cells (B). C: integrity of RNA of gastric, proximal small bowel, distal small bowel, and colon epithelial cell isolations was determined by the electrophoretic trace of the RNA sample. The software algorithm allows for the classification of eukaryotic total RNA, based on a numbering system from 1 to 10, with 1 being the most degraded profile and 10 being the most intact. RNA integrity number (RIN) for the four samples was 9.9–10.0. D: 3 additional, novel genes were validated by RT-qPCR and significant mRNA expression of carbonic anhydrases (CAH) 9, CAH11, and the flippase Atp10d was found in the gastric corpus epithelium. Abbreviations: DSB, distal small bowel; PSB, proximal small bowel. ***P < 0.001 vs. all other groups, ##P < 0.01 vs. stomach and proximal small bowel.